Glomalin Related Soil Protein (GRSP) is an insoluble, hydrophobic, proteinaceous extended hyphae combined with soil Arbuscular mycorrhizal (AM) fungi play an important role in the soil health and quality by improving the soil fertility, plant nutrition, soil aggregation and stabled soil structure. The efficacy and concentration of GRSP in soil to form extensive strong and thick mycelia association with soil particles like sand, silt and clay is mainly depends on soil AM fungal isolate and colonies. These AM fungal and GRSP association is directly affect and influence the soil aggregates and soil structure stability by hyphen envelopment of sand, silt and clay particles. This indicates the GRSP effect in soil aggregation and stability. Selection of good isolate of AM fungus from soil ecosystem plays a significant part in improvement of soil quality, health, and land reclamation and restoration activities.