Food safety is of major concern in any of the food processing industries as most of the freshly packed foods are subjected to microbial attack leading to growth of microorganisms. Growth of microbes leads to change in taste, smell, odour of packaged foods which is the huge loss for food industries as it is unfit for consumption. Therefore, there is need to identify and detect these microbes thus ensuring food safety. Several conventional methods are available, but all these methods are time consuming and laborious. But in recent years MALDI-TOF mass spectrometry is gaining popularity because it is more accurate, rapid method of microbe detection and identification.